diff --git a/tests/test_api.py b/tests/test_api.py
index 4d8ef1f9..76b8ba45 100755
--- a/tests/test_api.py
+++ b/tests/test_api.py
@@ -47,6 +47,7 @@
     import_ed25519_privatekey_from_file
 )
 
+from securesystemslib import hash as sslib_hash
 from securesystemslib.signer import (
     SSlibSigner,
     Signature
@@ -621,7 +622,49 @@ def  test_length_and_hash_validation(self):
             file1_targetfile.length = expected_length
             file1_targetfile.hashes = {'sha256': 'incorrecthash'}
             self.assertRaises(exceptions.LengthOrHashMismatchError,
-                file1_targetfile.verify_length_and_hashes, file1)
+                file1_targetfile.verify_length_and_hashes, file1)     
+
+    def test_targetfile_from_file(self):
+        # Test with an existing file and valid hash algorithm
+        file_path = os.path.join(self.repo_dir, 'targets', 'file1.txt')
+        targetfile_from_file = TargetFile.from_file(
+            file_path, file_path, ['sha256']
+        )
+
+        with open(file_path, "rb") as file:
+            targetfile_from_file.verify_length_and_hashes(file)
+
+        # Test with a non-existing file
+        file_path = os.path.join(self.repo_dir, 'targets', 'file123.txt')
+        self.assertRaises(
+            FileNotFoundError, 
+            TargetFile.from_file, 
+            file_path, 
+            file_path,
+            [sslib_hash.DEFAULT_HASH_ALGORITHM]
+        )
+
+        # Test with an unsupported algorithm
+        file_path = os.path.join(self.repo_dir, 'targets', 'file1.txt')
+        self.assertRaises(
+            exceptions.UnsupportedAlgorithmError,
+            TargetFile.from_file, 
+            file_path, 
+            file_path,
+            ['123']
+        )
+
+    def test_targetfile_from_data(self):
+        data = b"Inline test content"
+        target_file_path = os.path.join(self.repo_dir, 'targets', 'file1.txt')
+        
+        # Test with a valid hash algorithm
+        targetfile_from_data = TargetFile.from_data(target_file_path, data, ['sha256'])
+        targetfile_from_data.verify_length_and_hashes(data)
+
+        # Test with no algorithms specified
+        targetfile_from_data = TargetFile.from_data(target_file_path, data)
+        targetfile_from_data.verify_length_and_hashes(data)
 
     def test_is_delegated_role(self):
         # test path matches
diff --git a/tuf/api/metadata.py b/tuf/api/metadata.py
index fb4a1daf..cca82471 100644
--- a/tuf/api/metadata.py
+++ b/tuf/api/metadata.py
@@ -1219,6 +1219,78 @@ def to_dict(self) -> Dict[str, Any]:
             **self.unrecognized_fields,
         }
 
+    @classmethod
+    def from_file(
+        cls,
+        target_file_path: str,
+        local_path: str,
+        hash_algorithms: Optional[List[str]] = None,
+    ) -> "TargetFile":
+        """Creates TargetFile object from a file.
+        Arguments:
+            target_file_path: The TargetFile path.
+            local_path: The local path to the file to create TargetFile from.
+            hash_algorithms: An optional list of hash algorithms to create
+                the hashes with. If not specified the securesystemslib default
+                hash algorithm is used.
+        Raises:
+            FileNotFoundError: The file doesn't exist.
+            UnsupportedAlgorithmError: The hash algorithms list
+                contains an unsupported algorithm.
+        """
+        with open(local_path, "rb") as file:
+            return cls.from_data(target_file_path, file, hash_algorithms)
+
+    @classmethod
+    def from_data(
+        cls,
+        target_file_path: str,
+        data: Union[bytes, IO[bytes]],
+        hash_algorithms: Optional[List[str]] = None,
+    ) -> "TargetFile":
+        """Creates TargetFile object from bytes.
+        Arguments:
+            target_file_path: The TargetFile path.
+            data: The data to create TargetFile from.
+            hash_algorithms: An optional list of hash algorithms to create
+                the hashes with. If not specified the securesystemslib default
+                hash algorithm is used.
+        Raises:
+            UnsupportedAlgorithmError: The hash algorithms list
+                contains an unsupported algorithm.
+        """
+        if isinstance(data, bytes):
+            length = len(data)
+        else:
+            data.seek(0, io.SEEK_END)
+            length = data.tell()
+
+        hashes = {}
+
+        if hash_algorithms is None:
+            hash_algorithms = [sslib_hash.DEFAULT_HASH_ALGORITHM]
+
+        for algorithm in hash_algorithms:
+            try:
+                if isinstance(data, bytes):
+                    digest_object = sslib_hash.digest(algorithm)
+                    digest_object.update(data)
+                else:
+                    digest_object = sslib_hash.digest_fileobject(
+                        data, algorithm
+                    )
+            except (
+                sslib_exceptions.UnsupportedAlgorithmError,
+                sslib_exceptions.FormatError,
+            ) as e:
+                raise exceptions.UnsupportedAlgorithmError(
+                    f"Unsupported algorithm '{algorithm}'"
+                ) from e
+
+            hashes[algorithm] = digest_object.hexdigest()
+
+        return cls(length, hashes, target_file_path)
+
     def verify_length_and_hashes(self, data: Union[bytes, IO[bytes]]) -> None:
         """Verifies that length and hashes of "data" match expected values.