diff --git a/changes/issue-3399-last-used-column b/changes/issue-3399-last-used-column
new file mode 100644
index 0000000000..52f12251e7
--- /dev/null
+++ b/changes/issue-3399-last-used-column
@@ -0,0 +1 @@
+- Add last used column to host details' software table for Mac OS hosts
diff --git a/frontend/pages/hosts/details/HostDetailsPage/HostDetailsPage.tsx b/frontend/pages/hosts/details/HostDetailsPage/HostDetailsPage.tsx
index ed4846ad9f..2930f12228 100644
--- a/frontend/pages/hosts/details/HostDetailsPage/HostDetailsPage.tsx
+++ b/frontend/pages/hosts/details/HostDetailsPage/HostDetailsPage.tsx
@@ -596,6 +596,7 @@ const HostDetailsPage = ({
               isLoading={isLoadingHost}
               software={hostSoftware}
               softwareInventoryEnabled={hostSettings?.enable_software_inventory}
+              deviceType={host?.platform === "darwin" ? "macos" : ""}
             />
           </TabPanel>
           <TabPanel>
diff --git a/frontend/pages/hosts/details/cards/Software/Software.tsx b/frontend/pages/hosts/details/cards/Software/Software.tsx
index 8256d3c296..9229c9516b 100644
--- a/frontend/pages/hosts/details/cards/Software/Software.tsx
+++ b/frontend/pages/hosts/details/cards/Software/Software.tsx
@@ -23,6 +23,7 @@ interface ISoftwareTableProps {
   isLoading: boolean;
   software: ISoftware[];
   deviceUser?: boolean;
+  deviceType?: string;
   softwareInventoryEnabled?: boolean;
 }
 
@@ -30,6 +31,7 @@ const SoftwareTable = ({
   isLoading,
   software,
   deviceUser,
+  deviceType,
   softwareInventoryEnabled,
 }: ISoftwareTableProps): JSX.Element => {
   const tableSoftware: ITableSoftware[] = software.map((s) => {
@@ -104,27 +106,29 @@ const SoftwareTable = ({
             />
           )}
           {software && (
-            <TableContainer
-              columns={tableHeaders}
-              data={tableSoftware}
-              filters={filters}
-              isLoading={isLoading}
-              defaultSortHeader={"name"}
-              defaultSortDirection={"asc"}
-              inputPlaceHolder={
-                "Search software by name or vulnerabilities (CVEs)"
-              }
-              onQueryChange={onQueryChange}
-              resultsTitle={"software items"}
-              emptyComponent={EmptySoftwareSearch}
-              showMarkAllPages={false}
-              isAllPagesSelected={false}
-              searchable
-              customControl={renderVulnFilterDropdown}
-              isClientSidePagination
-              isClientSideFilter
-              highlightOnHover
-            />
+            <div className={deviceType || ""}>
+              <TableContainer
+                columns={tableHeaders}
+                data={tableSoftware}
+                filters={filters}
+                isLoading={isLoading}
+                defaultSortHeader={"name"}
+                defaultSortDirection={"asc"}
+                inputPlaceHolder={
+                  "Search software by name or vulnerabilities ( CVEs)"
+                }
+                onQueryChange={onQueryChange}
+                resultsTitle={"software items"}
+                emptyComponent={EmptySoftwareSearch}
+                showMarkAllPages={false}
+                isAllPagesSelected={false}
+                searchable
+                customControl={renderVulnFilterDropdown}
+                isClientSidePagination
+                isClientSideFilter
+                highlightOnHover
+              />
+            </div>
           )}
         </>
       ) : (
diff --git a/frontend/pages/hosts/details/cards/Software/SoftwareTableConfig.tsx b/frontend/pages/hosts/details/cards/Software/SoftwareTableConfig.tsx
index 3a7fb2f11d..995d3614d7 100644
--- a/frontend/pages/hosts/details/cards/Software/SoftwareTableConfig.tsx
+++ b/frontend/pages/hosts/details/cards/Software/SoftwareTableConfig.tsx
@@ -2,8 +2,7 @@ import React from "react";
 import { Link } from "react-router";
 import ReactTooltip from "react-tooltip";
 
-// TODO: Enable after backend has been updated to provide last_opened_at
-// import distanceInWordsToNow from "date-fns/distance_in_words_to_now";
+import { formatDistanceToNow } from "date-fns";
 
 import { ISoftware } from "interfaces/software";
 
@@ -25,6 +24,7 @@ interface ICellProps {
   };
   row: {
     original: ISoftware;
+    index: number;
   };
 }
 
@@ -40,6 +40,12 @@ interface IVulnCellProps extends ICellProps {
   };
 }
 
+interface ILastUsedCellProps extends ICellProps {
+  cell: {
+    value: string;
+  };
+}
+
 interface IDataColumn {
   title: string;
   Header: ((props: IHeaderProps) => JSX.Element) | string;
@@ -207,34 +213,54 @@ const generateSoftwareTableHeaders = (deviceUser = false): IDataColumn[] => {
         );
       },
     },
-    // TODO: Enable after backend has been updated to provide last_opened_at
-    // {
-    //   title: "Last used",
-    //   Header: (cellProps) => (
-    //     <HeaderCell
-    //       value={cellProps.column.title}
-    //       isSortedDesc={cellProps.column.isSortedDesc}
-    //     />
-    //   ),
-    //   accessor: "last_opened_at",
-    //   Cell: (cellProps) => {
-    //     const lastUsed = isNaN(Date.parse(cellProps.cell.value))
-    //       ? "Unavailable"
-    //       : `${distanceInWordsToNow(Date.parse(cellProps.cell.value))} ago`;
-    //     return (
-    //       <span
-    //         className={
-    //           lastUsed === "Unavailable"
-    //             ? "software-last-used-muted"
-    //             : "software-last-used"
-    //         }
-    //       >
-    //         {lastUsed}
-    //       </span>
-    //     );
-    //   },
-    //   sortType: "dateStrings",
-    // },
+    {
+      title: "Last used",
+      Header: (cellProps) => (
+        <HeaderCell
+          value={cellProps.column.title}
+          isSortedDesc={cellProps.column.isSortedDesc}
+        />
+      ),
+      accessor: "last_opened_at",
+      Cell: (cellProps: ILastUsedCellProps): JSX.Element => {
+        const lastUsed = cellProps.cell.value
+          ? `${formatDistanceToNow(Date.parse(cellProps.cell.value))} ago`
+          : "Unavailable";
+        const hasLastUsed = lastUsed !== "Unavailable";
+        return (
+          <>
+            <span
+              className={`last-used ${
+                lastUsed === "Unavailable" ? "text-muted" : ""
+              }`}
+              data-tip
+              data-for={`last_used__${cellProps.row.index}`}
+              data-tip-disable={hasLastUsed}
+            >
+              {lastUsed}
+            </span>
+            <ReactTooltip
+              place="top"
+              type="dark"
+              effect="solid"
+              backgroundColor="#3e4771"
+              id={`last_used__${cellProps.row.index}`}
+              className="last_used_tooltip"
+              data-tip-disable={hasLastUsed}
+              data-html
+            >
+              <span className={`last_used tooltip__tooltip-text`}>
+                Last used information <br />
+                is only available for the <br />
+                Application (macOS) <br />
+                software type.
+              </span>
+            </ReactTooltip>
+          </>
+        );
+      },
+      sortType: "dateStrings",
+    },
     {
       title: "",
       Header: "",
diff --git a/frontend/pages/hosts/details/cards/Software/_styles.scss b/frontend/pages/hosts/details/cards/Software/_styles.scss
index a191990a24..8da70f3558 100644
--- a/frontend/pages/hosts/details/cards/Software/_styles.scss
+++ b/frontend/pages/hosts/details/cards/Software/_styles.scss
@@ -1,4 +1,7 @@
 .section--software {
+  .text-muted {
+    color: $ui-fleet-black-50;
+  }
   .table-container__header-left {
     .controls {
       // vulnerable software dropdown filter
@@ -36,13 +39,16 @@
   }
 
   .data-table-block {
+    .last_used_tooltip {
+      text-align: center;
+    }
     .data-table__table {
       thead {
         .name__header {
           width: $col-md;
         }
         .version__header {
-          width: 0px;
+          width: $col-sm;
         }
         .source__header {
           display: none;
@@ -51,6 +57,9 @@
         .hosts_count__header {
           border-right: 0;
         }
+        .last_opened_at__header {
+          display: none;
+        }
         @media (min-width: $break-990) {
           .version__header {
             width: $col-md;
@@ -86,6 +95,9 @@
             }
           }
         }
+        .last_opened_at__cell {
+          display: none;
+        }
         @media (min-width: $break-1400) {
           .source__cell {
             display: table-cell;
@@ -131,7 +143,28 @@
       }
     }
   }
-  .text-muted {
-    color: $ui-fleet-black-50;
+
+  // Only show this column to macos users
+  .macos .data-table-block .data-table__table {
+    @media (min-width: $break-990) {
+      thead .version__header {
+        width: $col-sm;
+      }
+    }
+    @media (min-width: $break-1400) {
+      thead {
+        .vulnerabilities__header {
+          width: $col-md;
+        }
+        .last_opened_at__header {
+          display: table-cell;
+        }
+      }
+      tbody {
+        .last_opened_at__cell {
+          display: table-cell;
+        }
+      }
+    }
   }
 }
diff --git a/frontend/styles/var/tables.scss b/frontend/styles/var/tables.scss
index 22a3e61eaf..c03d8de21f 100644
--- a/frontend/styles/var/tables.scss
+++ b/frontend/styles/var/tables.scss
@@ -1,3 +1,3 @@
-$col-sm: 0px; // column size will "hug" its contents
+$col-sm: 62px; // column size will "hug" its contents
 $col-md: 202px; // column size not including 24px padding on left and right (total width is 250px)
 $col-lg: 352px; // column size not including 24px padding on left and right (total width is 250px)