diff --git a/packages/core/rxjs-interop/src/take_until_destroyed.ts b/packages/core/rxjs-interop/src/take_until_destroyed.ts
index db0e45d8fea..a72f2593cc4 100644
--- a/packages/core/rxjs-interop/src/take_until_destroyed.ts
+++ b/packages/core/rxjs-interop/src/take_until_destroyed.ts
@@ -26,8 +26,12 @@ export function takeUntilDestroyed<T>(destroyRef?: DestroyRef): MonoTypeOperator
     destroyRef = inject(DestroyRef);
   }
 
-  const destroyed$ = new Observable<void>((observer) => {
-    const unregisterFn = destroyRef!.onDestroy(observer.next.bind(observer));
+  const destroyed$ = new Observable<void>((subscriber) => {
+    if ((destroyRef as unknown as {destroyed: boolean}).destroyed) {
+      subscriber.next();
+      return;
+    }
+    const unregisterFn = destroyRef!.onDestroy(subscriber.next.bind(subscriber));
     return unregisterFn;
   });
 
diff --git a/packages/core/rxjs-interop/test/take_until_destroyed_spec.ts b/packages/core/rxjs-interop/test/take_until_destroyed_spec.ts
index 79c3c937fb7..59b85d6dbca 100644
--- a/packages/core/rxjs-interop/test/take_until_destroyed_spec.ts
+++ b/packages/core/rxjs-interop/test/take_until_destroyed_spec.ts
@@ -6,10 +6,18 @@
  * found in the LICENSE file at https://angular.dev/license
  */
 
-import {DestroyRef, EnvironmentInjector, Injector, runInInjectionContext} from '../../src/core';
-import {BehaviorSubject} from 'rxjs';
+import {
+  Component,
+  DestroyRef,
+  EnvironmentInjector,
+  inject,
+  Injector,
+  runInInjectionContext,
+} from '../../src/core';
+import {BehaviorSubject, finalize} from 'rxjs';
 
 import {takeUntilDestroyed} from '../src/take_until_destroyed';
+import {TestBed} from '@angular/core/testing';
 
 describe('takeUntilDestroyed', () => {
   it('should complete an observable when the current context is destroyed', () => {
@@ -76,4 +84,39 @@ describe('takeUntilDestroyed', () => {
 
     expect(unregisterFn).toHaveBeenCalled();
   });
+
+  // https://github.com/angular/angular/issues/54527
+  it('should unsubscribe after the current context has already been destroyed', async () => {
+    const recorder: any[] = [];
+
+    // Note that we need a "real" view for this test because, in other cases,
+    // `DestroyRef` would resolve to the root injector rather than to the
+    // `NodeInjectorDestroyRef`, where `lView` is used.
+    @Component({template: ''})
+    class TestComponent {
+      destroyRef = inject(DestroyRef);
+
+      source$ = new BehaviorSubject(0);
+
+      ngOnDestroy(): void {
+        Promise.resolve().then(() => {
+          this.source$
+            .pipe(
+              takeUntilDestroyed(this.destroyRef),
+              finalize(() => recorder.push('finalize()')),
+            )
+            .subscribe((value) => recorder.push(value));
+        });
+      }
+    }
+
+    const fixture = TestBed.createComponent(TestComponent);
+    fixture.destroy();
+
+    // Wait until the `ngOnDestroy` microtask is run.
+    await Promise.resolve();
+
+    // Ensure the `value` is not recorded, but unsubscribed immediately.
+    expect(recorder).toEqual(['finalize()']);
+  });
 });
diff --git a/packages/core/src/di/r3_injector.ts b/packages/core/src/di/r3_injector.ts
index 97e2fcf2a8e..96a6d227922 100644
--- a/packages/core/src/di/r3_injector.ts
+++ b/packages/core/src/di/r3_injector.ts
@@ -175,6 +175,9 @@ export abstract class EnvironmentInjector implements Injector {
 
   abstract destroy(): void;
 
+  /** @internal */
+  abstract get destroyed(): boolean;
+
   /**
    * @internal
    */
@@ -199,7 +202,7 @@ export class R3Injector extends EnvironmentInjector implements PrimitivesInjecto
   /**
    * Flag indicating that this injector was previously destroyed.
    */
-  get destroyed(): boolean {
+  override get destroyed(): boolean {
     return this._destroyed;
   }
   private _destroyed = false;
diff --git a/packages/core/src/linker/destroy_ref.ts b/packages/core/src/linker/destroy_ref.ts
index 08da24f0d59..86812ff4a3d 100644
--- a/packages/core/src/linker/destroy_ref.ts
+++ b/packages/core/src/linker/destroy_ref.ts
@@ -46,6 +46,9 @@ export abstract class DestroyRef {
    */
   abstract onDestroy(callback: () => void): () => void;
 
+  /** @internal */
+  abstract get destroyed(): boolean;
+
   /**
    * @internal
    * @nocollapse
@@ -64,6 +67,10 @@ export class NodeInjectorDestroyRef extends DestroyRef {
     super();
   }
 
+  override get destroyed() {
+    return isDestroyed(this._lView);
+  }
+
   override onDestroy(callback: () => void): () => void {
     const lView = this._lView;